FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204396
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGGA550.0144.020551
TGACTAG351.2732926E-11144.020551
GAAATCG100.0072424165143.950094
GAAGCGA100.0072424165143.950096
ACCGCGG151.2646697E-4143.950092
CGTTGCA151.2646697E-4143.950093
ACTTCGA202.2048298E-6143.950099
GTAGGGA151.2646697E-4143.950098
TAACGCG151.2646697E-4143.950099
ATCGTGG100.0072424165143.950097
TATGAAT100.0072424165143.950099
AGACGTA400.0143.950099
AGCGGAT306.82121E-10143.950098
TCGTGGG151.2646697E-4143.950098
GTTCGAG100.0072424165143.950096
TCAGACG400.0143.950097
TGTTACG100.0072424165143.950097
AGCGATA450.0143.950098
AGCGAAC151.2646697E-4143.950099
ATGGTGG100.0072424165143.950095
Produced by FastQC (version 0.11.5)