FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227362
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG650.0143.999771
AACGTAG253.8684448E-8143.936453
ACACGAA253.8684448E-8143.936457
CTATAGC202.2064105E-6143.936454
ATTACCG100.007245001143.936452
ACCGAGT100.007245001143.936454
AAAGTTG100.007245001143.936456
TAGTTAC100.007245001143.936457
TGGTTCG202.2064105E-6143.936454
CGGGGTA100.007245001143.936454
GAGCGCC202.2064105E-6143.936457
TTGACGT253.8684448E-8143.936459
TCGCTAG202.2064105E-6143.936458
ACGTCGC100.007245001143.936456
CCGAAGC100.007245001143.936454
TAAATAC100.007245001143.936459
TCGAACG100.007245001143.936458
TATCCGC100.007245001143.936456
TGTCCGA400.0143.936457
AAGTCGG100.007245001143.936456
Produced by FastQC (version 0.11.5)