FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127972
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA306.7484507E-10144.099331
CTGCCTA151.2583655E-4144.099331
ACGAGGA351.2732926E-11144.099331
GGCTCGA253.8326107E-8144.099331
ACGGCAG253.8506187E-8143.986723
CTTCGAA151.262293E-4143.986712
AGCAACG202.198858E-6143.986718
TCGTTGG100.007233769143.986716
TCGTTAA202.198858E-6143.986716
GTACCAT306.7848305E-10143.986718
GTATTAG151.262293E-4143.986718
CGTATAA100.007233769143.986717
TTGGACC100.007233769143.986719
CGACGGA151.262293E-4143.986719
GCGAAGT202.198858E-6143.986714
CCCTAGT100.007233769143.986713
TCACACT202.198858E-6143.986715
TAGGTCG100.007233769143.986714
CGGCAGA100.007233769143.986714
TAGCGAT100.007233769143.986719
Produced by FastQC (version 0.11.5)