FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121562
Sequences flagged as poor quality0
Sequence length8
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATACAA11965298.42878531119922No Hit
AGATTCAA1910.15712146888007766Illumina PCR Primer Index 8 (100% over 8bp)
AGATAAAA1910.15712146888007766RNA PCR Primer, Index 35 (100% over 8bp)
AGATACAT1710.14066895904970303Illumina PCR Primer Index 2 (100% over 8bp)
ACATACAA1620.13326532962603443No Hit
AGAAACAA1260.10365081193136014No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)