FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124575
Sequences flagged as poor quality0
Sequence length8
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATACAA12280998.58238009231387No Hit
AGATACAG2900.23279149106963676No Hit
AGATAAAA2050.1645595023078467RNA PCR Primer, Index 35 (100% over 8bp)
AGATACAT1490.11960666265302027Illumina PCR Primer Index 2 (100% over 8bp)
AGATTCAA1390.11157936985751556Illumina PCR Primer Index 8 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)