FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121921
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGC151.2626569E-4143.97216
ACTTGTA151.2626569E-4143.97212
ATGGCGC151.2626569E-4143.97212
ACGCAGG151.2626569E-4143.97214
CGACCAC151.2626569E-4143.97212
ATATGGC151.2626569E-4143.97212
CGATTGA306.7848305E-10143.97211
GCCGCAC151.2626569E-4143.97214
ACTACAA151.2626569E-4143.97212
ATAACGC306.7848305E-10143.97211
ATTGAAG151.2626569E-4143.97213
TCGCGCA151.2626569E-4143.97213
ACGAACA151.2626569E-4143.97213
AACGTTG202.1995438E-6143.972091
AGCCGCG100.007235545143.972092
TCGTTCG100.007235545143.972096
CGAACAA202.1995438E-6143.972094
CATATTC100.007235545143.972091
CTACTGT100.007235545143.972093
CCGATTC100.007235545143.972093
Produced by FastQC (version 0.11.5)