FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147455
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGCG450.0144.010861
CTTTGCG306.7848305E-10144.010851
TCCCGAT202.1981468E-6144.010851
TCGAGGC500.0144.010851
ACGAGCC253.849709E-8144.010851
CGGAAGC100.007238591143.962024
GGGTGCG100.007238591143.962026
GTCGCAT202.201863E-6143.962028
ACCGGAG100.007238591143.962023
GCGACGT100.007238591143.962023
ATGATCT100.007238591143.962024
ACGTTGA100.007238591143.962022
CCGATAG202.201863E-6143.962023
TAGACGA202.201863E-6143.962027
CGTTGTG100.007238591143.962024
TTAGAAG100.007238591143.962023
CGGCACG100.007238591143.962025
GGCAAGC100.007238591143.962026
GACCGGA100.007238591143.962029
CGCGCCA100.007238591143.962029
Produced by FastQC (version 0.11.5)