FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140114
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGT550.0143.992871
AAACTCG151.2623324E-4143.992864
AAGTACG253.851528E-8143.992867
GAGGACG100.007233574143.992869
AAATCCG100.007233574143.992869
GCGACTG100.007233574143.992862
GTTCTCG100.007233574143.992861
TCCGCGT151.2623324E-4143.992868
CGTATCG100.007233574143.992867
GTCGACT100.007233574143.992868
CGAGTAA100.007233574143.992865
ACTGATG202.199129E-6143.992861
CTATCCC100.007233574143.992869
CGTGCAT100.007233574143.992867
GAGTCGT253.851528E-8143.992863
CATTGGG100.007233574143.992868
GCACGTG151.2623324E-4143.992867
CACGCGA151.2623324E-4143.992868
AGGACGA151.2623324E-4143.992868
ATTCGTA151.2623324E-4143.992864
Produced by FastQC (version 0.11.5)