FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186931
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT400.0144.028371
CGATTGA400.0144.028371
CGTTAGA800.0144.028371
ATAACGC400.0144.028371
CGGACAG151.2644555E-4143.951314
AACGTGC151.2644555E-4143.951315
AATCCGA100.007241741143.951319
AGTTTCG100.007241741143.951317
GTCGACT151.2644555E-4143.951318
CTATCGA100.007241741143.951315
CTATAGA100.007241741143.951314
GGCGTAA202.2042386E-6143.951315
ACTCTAC202.2042386E-6143.951316
ACCTGTA100.007241741143.951314
CAATTGC151.2644555E-4143.951318
AGGCGAC202.2042386E-6143.951318
GCGTTGC100.007241741143.951319
TATTGAG450.0143.951313
AAACGTA151.2644555E-4143.951314
CTAACTG100.007241741143.951319
Produced by FastQC (version 0.11.5)