FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184833
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC550.0144.157961
GCATGAT750.0144.157961
TACCTTA450.0144.157941
CGTGTAA100.0072106775144.157941
AGCGTAC100.007239927143.962945
AGGTAAC100.007239927143.962946
TCGCAGA100.007239927143.962944
ACCGGCA100.007239927143.962943
CAACGAC100.007239927143.962942
ATGGCGA253.8613507E-8143.962942
ACGTTAT100.007239927143.962942
TAGCGTA100.007239927143.962949
GACGTAC100.007239927143.962945
GATCCGA100.007239927143.962946
CCGGAGG100.007239927143.962944
GTTAGTA202.2032855E-6143.962944
TTCCGGT202.2032855E-6143.962943
GCGTTGA100.007239927143.962946
TTATCGA100.007239927143.962945
GTTACTG100.007239927143.962948
Produced by FastQC (version 0.11.5)