FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180772
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATTG253.8584403E-8143.978711
CCGGTAG450.0143.97871
TTTACTC253.8648068E-8143.938879
GAATGGT151.2648223E-4143.938873
ATCCGGG151.2648223E-4143.938879
AAACGTC151.2648223E-4143.938874
CTAGTCG151.2648223E-4143.938874
ACAATAG151.2648223E-4143.938877
TCCGTAA151.2648223E-4143.938875
TAGTCGG151.2648223E-4143.938875
TAGTACG151.2648223E-4143.938877
CCGATTC202.204988E-6143.938863
TAAAGCG100.0072434177143.938865
CAACGAC100.0072434177143.938862
ATTACGA202.204988E-6143.938869
TTGTCGA100.0072434177143.938866
TGTATAG100.0072434177143.938867
TTGTACA100.0072434177143.938869
TTCAACA100.0072434177143.938867
CTAAGAG100.0072434177143.938865
Produced by FastQC (version 0.11.5)