FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178501
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGA202.2027725E-6143.966959
GTATTGC253.8600774E-8143.966952
CGAGTAA100.0072391178143.966955
GCGAAGT202.2027725E-6143.966954
GCGCGAT253.8600774E-8143.966951
TAGGTCC202.2027725E-6143.966955
CGTTGGA100.0072391178143.966957
TTCACGA100.0072391178143.966956
TGTATAT100.0072391178143.966957
TTGTAAA100.0072391178143.966956
TAAGTAT100.0072391178143.966955
CGATTCC100.0072391178143.966954
CGTTCTA100.0072391178143.966955
TAACGCA100.0072391178143.966952
TTATCGA100.0072391178143.966955
GTTACGG100.0072391178143.966959
CCGTAAG100.0072391178143.966952
TCTACGG100.0072391178143.966952
GATGGTA100.0072391178143.966954
TTGAACG650.0143.966951
Produced by FastQC (version 0.11.5)