FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176506
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGA253.8568032E-8143.986112
GACGTCG100.007236168143.986112
ATTCGCG100.007236168143.986112
ACGGGCG100.007236168143.986112
CCTTTAA151.2631193E-4143.986112
GACGGCG151.2631193E-4143.986112
CATACGA100.007236168143.986112
GATTGAC151.2631193E-4143.986112
AGCTAAT100.007236168143.986112
CTGATAG450.0143.986111
CCCATAC100.007236168143.986111
CTTCGTA306.8030204E-10143.945334
AACGTAC100.0072423015143.945335
TCGTTAG100.0072423015143.945336
TCGTTAC202.2043496E-6143.945338
GAAGCGT100.0072423015143.945336
TCACCCG100.0072423015143.945338
TCCGCAA253.8633516E-8143.945338
CAACGTA100.0072423015143.945334
GGTCGAG151.2645463E-4143.945337
Produced by FastQC (version 0.11.5)