FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180503
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCC151.262391E-4144.008322
TAACGCC151.262391E-4144.008322
TGCGGAG351.2732926E-11144.008322
TGCGGAA151.262391E-4144.008322
GAATAGA202.1996912E-6144.00832
CACTACC100.007232969144.00832
GCGGGTG100.007232969144.00832
GCGTGGA202.1996912E-6144.00832
AAACTAG100.007238964143.968413
CGGAAGT253.8600774E-8143.968414
GTCGCTA151.2637855E-4143.968417
AATCCGA253.8600774E-8143.968419
ACACGGA100.007238964143.968417
GTCGACT151.2637855E-4143.968418
GACACTC151.2637855E-4143.968413
CTATCGC100.007238964143.968415
TAGGTTA202.2027289E-6143.968415
TTGCTCG151.2637855E-4143.968414
TAGGTGG100.007238964143.968417
GGCGTAA100.007238964143.968415
Produced by FastQC (version 0.11.5)