FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597991
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG4800.0112.5138851
GTACGAT3150.0107.421659
ATTACGA2500.0106.553159
GACGCGT6800.0105.8954241
CCTTACG4450.0105.1815261
TCGCTAG2150.0103.8072748
CGTCTAA2850.0103.572297
TGACGGC4800.0103.512781
TCGAATG4800.0101.993448
CGTTAGA5600.0101.583971
TTGAACG5400.0101.345841
CGATTGA4350.0100.9779741
TCGTTAC5650.0100.666098
CCTTCGA6850.098.815121
CGCTATC4750.098.538481
GTGCATA6950.098.429411
TCGCATG4650.097.5421148
CGTTGGG6650.097.455641
GCGCGAT5050.096.962461
GATCGTA4700.096.522551
Produced by FastQC (version 0.11.5)