FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246402
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT4900.0113.2088241
CGATTGA3450.0112.761371
CCTTCGA4900.0108.7980961
TCGTTAC4800.0107.997958
CGTTAGA5400.0106.728871
TGACTAG5200.0106.677561
CGGACTG3400.0103.825221
CCTTACG3350.0103.2243351
TTTATGC5250.0101.544891
GTCGTAA4000.0100.858791
CGCTATC3500.0100.858781
TCGAATG3150.0100.569538
TGCGTGG3800.0100.4796141
TATGCCC4850.099.521921
AATCGGT5800.099.368251
TTACGCG1450.099.3084649
TACACGA5100.098.8811651
CACGATC3750.097.9495853
ATTACGA2500.097.9181449
GCGCGAT3900.097.903211
Produced by FastQC (version 0.11.5)