FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253006
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA3100.0113.845321
TGACGGC3200.0112.5384141
CCTTCGA4650.0109.973031
CCTTACG3250.0108.590921
GACGCGT4800.0108.036891
TCGCTAG1400.0107.9980858
CCGGTAG2500.0106.59641
GTCGACT2850.0106.1033868
GTCGTAA3750.0105.636061
TTAGGCA5050.0105.5409851
CCGAATG2050.0105.363989
TACACGA4250.0105.071171
TGACTAG3800.0104.246121
ACACGAT2500.0103.678162
CGGACTG3550.0103.471951
CGTTAGA4200.0102.892271
CGCTATC3850.0102.8922651
GGGCGTA1350.0101.3315352
TGCGTGG3600.0100.034151
AACCGCG3600.098.033461
Produced by FastQC (version 0.11.5)