FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284510
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC432083.3637729562245524No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC266422.0740982942912085No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC154541.2031046858335086No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC90820.7070400386139462No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC78780.6133077983044118No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC49960.3889420868658088No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT44460.3461242030034799No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT26950.20980763092541121No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG18530.1442573432670824No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14340.11163790083378099No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG12940.10073880312337No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG12900.10042740033164396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG40050.0101.8920751
GTCTCTC37900.095.898552
TCTCTCA40650.089.952613
CTGATGG128200.085.164271
GGCTCAA134650.080.572186
GCTCAAA134800.080.375717
CTCAAAC134850.080.2956548
TCAAACA139800.077.152599
TGGCTCA140800.077.0498665
GATGGCT145200.075.202033
TCAGCTG48600.073.916697
AGCTGGT49000.073.765549
CTCTCAG51500.071.006964
AGTCTCT53000.069.2554861
TGATGGC160500.068.428872
TGATACG755.140464E-967.1811453
ATGGCTC163550.066.901744
CTCAGCT55000.065.969946
CAGCTGG56250.064.6344458
TCTCAGC57700.063.2548035
Produced by FastQC (version 0.11.5)