FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282983
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT5900.0119.665581
CGGACTG3650.0114.480181
GTGCATA4550.0110.836241
CGATTGA3750.0107.585051
GCGCGAT3900.0107.14171
CCTTCGA4900.0105.8599241
CACGATC3150.0105.181863
TCGAATG3150.0105.140878
TCGACTA1450.0104.273889
CCTTACG3250.0104.1860661
GACCAAT5400.0104.062921
TACACGA4900.0102.919371
CGCTATC3600.0102.061711
TGACTAG4100.0101.915281
TTTATGC5000.099.420111
CGCGGTA4350.099.370431
CGTTAGA4650.099.156731
TCGTTAC4300.098.7888268
ATTACGA1750.098.740999
CGTAGCG5550.098.654241
Produced by FastQC (version 0.11.5)