FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316335
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA2350.0113.355969
TGACTAG3650.0110.573561
TCGTTAC4950.0109.085398
CGGACTG4100.0108.9843141
GACGCGT5850.0108.41341
CACGATC3700.0107.107043
CGATTGA3450.0106.5386661
TTGAACG4650.0102.293281
GCGCAAC4050.0101.432231
TACACGA6200.0101.130851
TAGTACG2650.0100.523217
TGACGGC3300.0100.4615861
ACACGAT3400.099.618972
GCGACTA1550.097.636162
AGCGTTG1850.097.292378
TGCGTGG4300.097.211061
TATGCCC4600.097.138191
CCTTCGA6100.096.881351
CCTTACG3800.096.725891
CGTTAGA4250.096.658951
Produced by FastQC (version 0.11.5)