FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316335
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC434023.2971849871043464No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC262221.9920460976878984No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC153101.1630777879491163No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC90500.6875149562991184No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC81690.6205867047522098No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC47620.36176201346921566No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT44240.3360846593002541No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT26520.20146847117185215No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22270.16918185720200407No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG19220.14601146364717188No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG38900.099.051271
GTCTCTC40700.094.75752
TCTCTCA41750.091.512673
CTGATGG135650.087.7094041
CTCAAAC139200.084.851818
GCTCAAA138850.084.800067
GGCTCAA139900.084.160416
TCAAACA142550.082.510579
TGGCTCA149150.079.227445
GATGGCT152900.077.504933
TCAGCTG50350.076.3309257
AGCTGGT51400.075.903349
AGTCTCT53150.073.578761
CTTCCGA1500.071.957534
TGATGGC166850.071.197392
CTCTCAG55000.069.864224
ATGGCTC171250.069.625474
CTCAGCT56700.068.668326
CAGCTGG58450.066.373698
CGATCTA554.2407864E-665.4333348
Produced by FastQC (version 0.11.5)