FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319343
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3550.0115.616981
CGGACGT3550.0115.616981
GACGCGT5650.0114.701531
TCGCTAG1950.0110.771718
TGACTAG3850.0108.478171
TACACGA5350.0107.6741261
GTACGAT2100.0106.280049
GCGCGAT4050.0104.899191
CGATTGA3100.0104.52641
TAGGCTA2450.0102.859447
ATTACGA1750.0102.851659
CTTGACG2750.0102.103639
ACGAGCC4100.0101.8636551
CACGATC3900.0101.5522843
AGTATTG3100.099.873218
TCGCGAG3750.099.849811
TCGTTAC5000.099.362228
ACGATAC5050.098.38591
TTAGGCA4250.098.268461
TCGAATG3600.098.002198
Produced by FastQC (version 0.11.5)