FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700762
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATA3550.0121.749871
CTAATGG1750.0119.372841
TCGAATG1900.0117.472658
TGACTAG1650.0113.510221
CGGACGT1350.0112.054961
CACGATC1800.0111.9993
GCGTACC900.0111.9996
TAGCGTG850.0110.116669
GACGCGT3600.0110.053981
TCGTTAC2900.0109.24048
ACTAGGT2400.0107.999043
CGGACTG2550.0107.346771
TTAGGCA2350.0107.2866741
GATCTAG1150.0106.4870151
TTACGCG750.0105.599069
TTTGCGC2550.0104.521861
CCGGTAG1750.0102.907621
GATCGTA2400.0102.050061
GGCGTAA502.055458E-10100.7990955
CTAATAG1000.0100.7990953
Produced by FastQC (version 0.11.5)