FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559594
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC546513.5041812163934973No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC337772.165755959563835No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC194961.250068928195415No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC115300.73929497035767No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC101850.6530545770245333No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC64350.4126073837165314No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT56430.36182493648988134No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT34490.221147298591813No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG27880.17876447331805584No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17130.10983627790309529No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG15990.10252668322653204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG48000.0101.016841
GTCTCTC46200.099.2492752
TCTCTCA49750.092.1612553
CTGATGG162300.086.874481
GGCTCAA169200.083.2117166
GCTCAAA169550.083.0001457
CTCAAAC171600.082.13978
TCAAACA176350.079.968099
TGGCTCA177700.079.028885
TACGGCG756.002665E-1176.779396
GATGGCT183750.076.620253
AGCTGGT61550.074.6191569
TCAGCTG62100.073.953547
ATACGGC801.05501385E-1071.980685
AGTCTCT64250.071.318791
TGATGGC200650.070.278942
CTCTCAG65650.069.6211244
ATGGCTC203850.069.312514
CTCAGCT67800.068.15876
CAGCTGG70050.065.461968
Produced by FastQC (version 0.11.5)