FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596591
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA3350.0118.2767641
CGGACTG4250.0115.266081
ATTACGA2500.0112.310539
CCTTACG5200.0109.447361
TGACTAG6100.0106.290441
GCGCGAT4750.0106.166121
CCTTCGA6250.0106.04481
TGTACGC2050.0105.356967
TCGCTAG2000.0104.391198
CACGATC4150.0104.152843
GTACGAT3050.0103.860099
ATAACGC3750.0103.739471
TGACGGC5100.0103.117951
TCGTTAC5350.0102.271758
GACGCGT5750.0101.484271
AATCGGT7400.0100.2737051
TTTGCGC6200.099.928261
TAGCCGG5500.099.547981
TTAGGCA5900.098.904171
ACACGAT3650.098.686722
Produced by FastQC (version 0.11.5)