FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934979
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTGG3100.0116.213191
TTGAACG2850.0111.238451
TCCCGAT4000.0108.078261
TCGAATG2200.0107.9973458
GTGCATA3750.0107.597921
ACGAGGA2750.0107.4232561
GCGTCTA2050.0105.3632669
CGCTATC2400.0105.07611
ACGGAGT2000.0104.453293
GACCAAT3750.0103.755141
TTTAGAC1250.0103.732935
CATCGAA1950.0103.459551
TTCGAAG3800.0102.3899461
TGACTAG1700.0101.7207261
CGAACGG1500.0100.797539
GCATGCG2150.0100.5379261
AGGTCCG3450.0100.246511
ACGATAC2950.0100.140321
TAGGCTA1950.099.6898657
CCCGATA1750.098.803842
Produced by FastQC (version 0.11.5)