FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961457
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC3150.0118.959061
TCGAATG2400.0113.990948
CCCGATA1300.0110.858442
ACGCCGA3850.0110.432271
CGCTTCA2550.0110.212061
TTGAACG2900.0109.335041
TGACTAG2250.0108.893291
TTCGAAG3000.0108.0926061
GTAAACG1000.0107.9914258
AGCGATA1950.0107.068418
CGGACTG2900.0106.850151
GATGCGA1550.0106.830229
TAGACTA2100.0106.277277
CGAACAT5300.0106.0531161
GATCTAG3750.0105.690551
TGCGAAA750.0105.591619
TAACGTG1100.0104.8116152
CGATACG1450.0104.359832
ACGATAC3250.0104.212361
GCCGAAG2700.0104.0729143
Produced by FastQC (version 0.11.5)