FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962609
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATGT2800.0118.3325351
CCCGATA1350.0117.3675842
CGCTATC3750.0113.324851
TGACTAG2100.0113.1876451
AGCGATA2800.0113.140618
TCGAATG1800.0111.997778
TTCGAAG3250.0110.813081
TCCCGAT4300.0108.8802951
GTTTGAC2050.0108.8758858
TTTGCGA600.0108.0315252
ATACGTA1200.0108.025914
GCAGTTA3400.0105.924261
TACGGAT2600.0105.272421
AGCGTAT2700.0104.041171
TTACGCG900.0103.997939
TAGTTGG2150.0103.855061
TTTAGAC1250.0103.704875
TACACGA4250.0103.3820951
GACCAAT4250.0101.687311
TAGGCTA2200.0101.452547
Produced by FastQC (version 0.11.5)