FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027360
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG2450.0123.511851
CGCTATC4700.0114.971131
CGAGCTA1150.0112.6948556
CCTTACG2650.0111.47141
TAGCGTG1500.0110.399229
CCGGTAG3500.0109.1021351
CGATTGA2400.0105.070841
GTCGTAA2750.0104.7979351
ACGAGGA3300.0104.7979351
GTAGGGA1100.0104.726538
AACCGCG3950.0103.9688341
TAGTTGG3500.0102.9265441
TACGGAT3600.0102.068821
AGCGATA2400.0101.999278
TCGAATG2400.0101.999278
GGTATTG4250.0101.7156451
CTGATAG3050.0101.576691
TCGCGAG2850.0101.120811
CGAACGG1500.0100.7992869
TGAGCCG3450.0100.241511
Produced by FastQC (version 0.11.5)