FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052880
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAAG3200.0112.5328451
CCTTACG2050.0112.423061
CGCTATC3550.0111.5818561
TTACGGA850.0110.113189
GATCGCG3100.0106.86991
ATGCGAT2900.0106.7897951
TAGACTA2500.0106.560747
TAGTTGG3000.0105.630831
TATTAGC1100.0104.723029
CGATTGA2000.0104.430481
TACGGAT4000.0102.629961
AGCGATA2900.0101.793818
ATACGTA1350.0101.3532944
GACCGGA1500.0100.7959069
ACGATGT3950.0100.282431
CGGACGT2100.099.4575961
TCGAATG2250.099.200688
GACCAAT3850.099.145831
CGTTAGA3200.099.028911
TTCGTAA800.099.019495
Produced by FastQC (version 0.11.5)