FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052880
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC402173.8197135476027655No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC165821.5749183192766507No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC126011.196812552237672No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC75250.7147063293062837No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC50610.48068155915204014No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT40250.38228478079173317No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC31970.3036433401717195No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT16710.15870754501937542No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG15030.14275131069067698No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13060.1240407263885723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCTC39800.099.259762
TCTGATG28400.096.2829061
TCTCTCA42150.092.87653
AGTCTCT44500.089.099551
CTGATGG103150.087.0621261
CTCAAAC108400.082.150468
TCAGCTG48650.081.378227
GGCTCAA109250.081.367916
GCTCAAA110800.080.3633967
AGCTGGT49450.079.9464959
CTCTCAG50400.078.394874
TCAAACA113550.078.256689
GATGGCT115350.077.296223
TGGCTCA116650.076.07915
CTCAGCT52400.075.825546
CAGCTGG54100.073.4533168
ATGGCTC121550.073.182854
TCTCAGC56400.070.061645
TGATGGC129200.069.5081942
TCCGATC1102.7284841E-1158.8921626
Produced by FastQC (version 0.11.5)