FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924189
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC2850.0118.824131
TAGTTGG3150.0114.369721
TAGACTA2250.0108.797517
AGCGATA2250.0108.797518
GTCGTAA3450.0108.601521
GGTATTG4100.0107.200691
TCGCGAA950.0106.102839
AACCGCG3350.0105.390851
TAAACCG2600.0105.308131
ACGAGGA2750.0104.804271
CCTTACG2650.0103.3211751
TATGCCC3350.0103.240011
TTCGAAG3350.0103.240011
TGACTAG2250.0102.475271
AAGCTAA1700.0101.644737
CTAATGG1500.0100.87411
GATCTAG5000.0100.874091
TAGGCTA2000.0100.797697
GCATGCG1650.0100.437411
TCCCGAT3450.0100.247551
Produced by FastQC (version 0.11.5)