FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271905
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC463463.6438256001824034No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC193011.5174875482052512No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC146151.149063805866004No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC86040.6764656165358262No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC59640.4689029447954053No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT46970.3692885868048321No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC36460.2866566292293843No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT19020.14953947032207593No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG17400.13680267001073193No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17320.13617369221757913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCTC43550.098.0422742
TCTGATG32300.096.6982351
TCTCTCA46500.091.635123
CTGATGG120450.086.236051
CTCAAAC125150.083.0480658
GGCTCAA127350.081.782946
GCTCAAA127600.081.340677
AGTCTCT52900.080.859711
TCAAACA131200.079.334449
TGGCTCA133650.077.604745
GATGGCT134100.077.1296163
AGCTGGT55350.076.7293859
ATGGCTC140250.074.004074
TCAGCTG57350.073.7967157
CTCTCAG58300.073.0880364
CTCAGCT59600.070.890016
TGATGGC152050.068.237712
CAGCTGG62150.067.981418
TCTCAGC66650.063.49955
TCCGATC1650.047.98466
Produced by FastQC (version 0.11.5)