FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932637
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT2350.0125.6678851
CGTAACA1900.0113.681463
GCAGTTA3250.0113.030361
CGCTATC3450.0112.741281
GTGCATA3400.0112.280731
AGCGATA2900.0109.238738
TAGGCTA2650.0108.676627
TCGAATG2000.0107.997398
GCGATAT1400.0107.997389
CGCTTCA2750.0107.388911
GCGCTTA950.0106.10279
TTGAACG2400.0105.042511
TATTGAG2000.0104.397483
ACGAGGA2450.0102.8987961
GCGAAGT700.0102.854654
GATCTAG3300.0102.586971
TAGCCGG2750.0102.150431
CGAATGT4200.0101.1838151
TAGTTGG3000.0100.840811
GCATGCG1950.099.7326661
Produced by FastQC (version 0.11.5)