FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932637
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC365233.91610026194543No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC151941.6291440292418162No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC112881.2103315652284865No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC69060.740481023163353No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC45550.48840009564278497No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT37050.39726067055027836No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC29070.3116968338163723No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT15390.1650159708439618No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG13610.14593030300106044No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11550.12384239527275884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG25100.0105.894831
GTCTCTC33950.093.2893752
TCTCTCA34700.091.268143
CTGATGG91600.086.186091
AGTCTCT37550.084.2125241
CTCAAAC98050.080.758398
GGCTCAA97900.080.657216
GCTCAAA98700.080.007757
TCCGATC1100.078.524996
TGGCTCA101500.077.725555
GATGGCT102150.077.438213
TCAAACA103200.076.72839
ATGGCTC105800.074.766664
AGCTGGT42450.073.426499
CTCTCAG43750.072.388674
CGATCTA406.9024165E-571.985098
TTCCGAT1200.071.981235
TCAGCTG43700.071.161477
CTCAGCT44350.070.763966
CTTCCGA1250.069.098284
Produced by FastQC (version 0.11.5)