FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954342
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC3950.0114.956371
AGCGTAT2350.0113.481081
TTCGAAG3250.0113.103591
ACGAGGA3200.0110.36611
CCTTACG1850.0109.087721
GGTATTG4500.0108.914581
CTACTTA2450.0105.79089
CATCGAA3850.0102.9654541
TATTGAG2200.0101.5453953
TCCCGAT3550.0101.515241
CTTTGCG2700.0101.440051
GTCGTAA3300.0100.469331
CGATCGG3450.0100.27941
ACGATGT3750.099.945141
GATCTAG4050.099.6603851
AGCGATA2900.099.3055348
CGAGCTA1600.099.088666
TCGAATG2700.098.661898
GCGTATA1700.097.4996
CGGCTAG2900.096.929551
Produced by FastQC (version 0.11.5)