FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954044
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAAG3150.0123.435891
ACGCCGA3800.0121.2703551
GTGCATA3050.0115.678991
CGCTATC3500.0115.206831
TACGGTA2850.0111.164491
CCTTACG2400.0111.0065841
CTTTGCG2750.0109.970151
CCGTAGG1200.0107.9950949
ACGAGGA2750.0107.351821
TCGCGAG2250.0105.606261
TGACTAG2250.0105.606261
TCCCGAT3650.0104.5541461
CGATTGA3000.0103.206121
AGCGTAT2100.0102.863241
ACGATGT3100.0102.199611
TAGGCTA2050.0101.849037
GCCGACA2550.0101.653083
TCGAATG2200.0101.4499368
TAGTTGG2700.0101.339341
TTGAACG4000.0100.8059841
Produced by FastQC (version 0.11.5)