FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628966
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC198883.1620151168743624No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC88051.3999166886604364No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC63481.0092755411262293No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC37150.5906519589294175No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC28170.4478779457077171No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT20670.3286346161795709No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC17050.2710798357939857No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT9220.14658979976660105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCTC20500.091.281932
TCTGATG16950.090.932741
TCTCTCA21750.086.3598863
TACGGCG353.5616657E-582.260586
CTGATGG56550.081.130291
AGTCTCT23450.079.855841
GCTCAAA57350.079.4456567
CTCAAAC57450.079.194688
GGCTCAA57550.078.9194266
TGGCTCA58700.077.373315
TCAAACA59100.077.105479
AGCTGGT25150.075.567479
GATGGCT60400.075.421913
TCAGCTG25050.074.995067
ATGGCTC62250.073.30194
CTCTCAG26200.071.6975864
CTCAGCT27150.069.194336
TGATGGC67200.068.009532
CAGCTGG30350.061.9085668
TGATACG350.003671744861.6856353
Produced by FastQC (version 0.11.5)