FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642489
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA2350.0119.501321
CCTTACG1600.0117.01171
ATGCGAT2650.0114.124621
TGGTTAG2550.0112.952471
CGTGCAA1350.0112.011217
CGATTGA1500.0110.411041
CGCTATC2650.0108.690121
AGCGATA1250.0103.690368
AACCGCG2700.0101.3434751
GCGCGAT1650.0100.373671
AGCGTAT2250.099.2099151
GATCGTA2050.098.35131
CGTCTAC1400.097.724061
TACGGAT1850.097.307021
GAGTAGT750.096.024554
TAGTTGG2400.096.0096051
ACGATAC1650.096.00961
CTCTACG2250.096.00961
TCGAATG1650.096.00968
TACGGTA1750.094.638031
Produced by FastQC (version 0.11.5)