FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642489
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC191372.9785723957919905No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC86091.3399451196829828No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC60960.9488100185372824No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC36780.5724611627592068No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC27070.42133017063327155No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT20130.3133127571055691No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC15780.2456073177906548No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT9210.14334875772192207No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6580.10241420475681295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG15800.094.291991
GTCTCTC21300.090.893792
TCTCTCA22250.086.3663
TACGGCG353.561907E-582.2597356
CTGATGG53900.081.852751
CTCAAAC54700.080.931378
AGTCTCT23900.080.704631
GGCTCAA54800.080.252026
GCTCAAA55000.079.9602057
TCAAACA57300.077.145499
TGGCTCA57350.076.809225
GATGGCT57550.076.661393
ATGGCTC60450.072.989354
CTCTCAG26700.071.9772644
AGCTGGT26500.070.6357049
TCAGCTG26750.069.690137
TGATGGC66200.066.86192
CTCAGCT28400.066.401566
TCTCAGC30650.062.4664045
CAGCTGG30500.062.0704778
Produced by FastQC (version 0.11.5)