FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105255
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC323852.9300930554487428No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC145661.3178859177293927No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC105500.9545308548705954No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC61530.5567041090065189No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC45080.4078696771333312No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT33940.3070784570076589No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC27780.2513447123062099No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT15750.14250105179347752No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12680.1147246563010346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCTC31750.091.613712
TCTGATG26400.090.956141
CTCAAAC93000.083.5694358
CTGATGG93400.083.381281
GCTCAAA93950.082.4142767
TCTCTCA35300.081.9483
GGCTCAA94500.081.934626
TCAAACA96950.080.75849
GATGGCT99300.077.39433
TGGCTCA99550.077.344515
AGTCTCT39150.075.516831
ATGGCTC103000.074.8237154
TCCGATC1650.074.139896
CTCTCAG42150.068.971674
AGCTGGT42150.068.804069
TCAGCTG42200.068.548927
TGATGGC115100.066.994162
CTCAGCT44150.065.521286
CCGATCT2000.061.1654137
CAGCTGG50150.058.115338
Produced by FastQC (version 0.11.5)