FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134072
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC4400.0113.030471
GCGCGAT2350.0110.416441
TACGGTA3400.0110.236021
ATTACGA1450.0109.233459
ATGCGAT3150.0107.544051
CGTCTAC3550.0105.578161
CTTACCG4750.0103.1844941
CCTTACG3750.0101.869391
GTCGACT2250.099.19288
CTTTGCG3050.096.890931
CTCGAAA4100.096.68921
TACGGAT2850.096.10321
TAGTTGG3450.096.10321
GATCGTA3350.094.668821
CGTTGGG3900.094.255061
GACCAAT5050.094.200171
AGCGTAT3700.093.505811
ACGATAC4650.093.00311
CGATCGG4500.091.298041
CGTTAGA4150.090.313851
Produced by FastQC (version 0.11.5)