FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135231
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA4700.0113.3717961
CGGACTG3550.0107.502531
GATCGTA3050.0103.8781051
CGATTGA2500.0103.689231
TAGTTGG4250.0101.65611
CCTTACG3500.0100.8089751
TCCCGAT3150.0100.580381
CGTTGGG3600.0100.00891
TACGGAT3600.0100.00891
TACGGTA4200.097.7229841
CGCTATC3550.097.360781
TCGTTAC2300.097.039298
ATGCGAT4150.095.430181
ACGATAC3250.095.270021
TGTACCG2600.094.149797
GCGCGAT2700.093.3416441
TATTACC4250.093.184761
AACGTTG4050.092.4526751
CCGGTAG2500.092.1682051
TCAACGA4150.091.959991
Produced by FastQC (version 0.11.5)