FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135231
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC320152.820130880851562No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC145251.279475278599686No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC103220.9092422599453327No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC59850.5272054762422802No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC46340.408198859967707No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT32520.28646152192813623No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC27480.24206527129720734No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT15310.1348624200713335No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12160.10711476342700296No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG25200.098.522861
GTCTCTC35350.091.0032
TCTCTCA37400.086.792213
CTGATGG90700.081.723771
CTCAAAC93600.079.607718
GGCTCAA93550.079.181546
GCTCAAA94050.078.913677
AGTCTCT42150.076.3182451
TCAAACA97900.075.970779
GATGGCT99150.074.768783
TGGCTCA99150.074.4882665
CTCTCAG45100.071.8144454
ATGGCTC103700.071.5575944
TCAGCTG45750.070.806617
AGCTGGT45700.070.739039
CTCAGCT48350.066.850136
TGATGGC112000.066.313092
CAGCTGG53600.060.4419368
TCTCAGC55500.058.3650325
TGATACG752.6629881E-547.9826853
Produced by FastQC (version 0.11.5)