FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879352
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT2800.0126.1422041
CCTTACG2800.0110.696221
AACCGCG3650.0110.590431
TACGGGC2650.0103.361811
CGTTAGA3350.0103.280621
CGCTATC3700.0103.251541
GATGCGA1050.0102.850289
CGTTGGG2900.0101.9081
CTTACCG3700.0101.30341
CGGACTG2000.0100.9137651
GATCTAG2650.0100.641761
TTGAACG1900.098.637521
AGTACGA2200.098.175278
ACGCGAA1550.097.541889
CGATTGA2900.096.936881
TGACTAG2850.096.108361
TAGTTGG3750.096.108351
ACCGCTA2100.095.99369
AGCGTAT2200.095.016211
CGATCGG3200.094.606661
Produced by FastQC (version 0.11.5)