FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978305
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA4650.0111.5151441
CGCTATC3050.0106.25931
CGATTGA3000.0105.6296161
TACGGTA2650.0103.274251
CTAATGG3400.0101.6755751
CCTTCGA3300.098.209361
CACCGAA2750.096.8701868
ATGCGAT2850.096.0269241
GTCGAAA750.095.9974758
TCAACGA3700.095.378091
GATTACG1450.094.342352
GTGCATA2750.094.280981
CGACTTC3900.094.180261
CGGACTG2950.092.771781
ATCCGAA2800.092.597391
CGTTGGG3200.092.275871
GACCAAT3600.090.0252461
TCGCATG2800.089.997648
TGACTAG3450.089.76431
ACGATAC4350.089.404381
Produced by FastQC (version 0.11.5)