FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001260
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC3500.0107.142771
GCGCGAT2500.0106.730691
CTTACCG3450.0102.424671
TCGTTAC1900.0102.305688
TAGCGAG850.0101.758973
CGGACTG4300.0100.6260451
CTCGAAA3700.099.402211
TACGGGC3350.099.0240251
TCCACGA3650.098.620398
CGTTAGA4400.096.7000961
TATGCCC4150.095.574531
CGATTGA2150.093.917641
TAAACCG1700.093.3257141
GACGCGT3400.093.3257141
GTACGTA3350.092.565941
ACGATAC3200.092.397761
TCGCGAG2500.092.3076251
GCGCAAC3850.091.783141
AACCGCG3800.091.093041
CCCGATA950.091.083932
Produced by FastQC (version 0.11.5)