FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7657796
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC46000.029.67253941
ATAGTAC47050.029.08435240
TACCTCG47900.028.34904916
TTAATCA75500.028.3235638
ATTAATC74050.027.271217
AATAGTA56050.027.22407539
ATTACCT67150.026.63299414
GTACCAA52050.026.4925343
GTCCTAT62900.026.3739851
AAATAGT63850.025.21615838
GGTCCTA54600.025.19081350
TACCAAG55400.025.14324244
TCATTAC87300.024.9361712
CATTACC73400.024.84233913
TGCTAAT62250.024.80034670
CATGCTA67000.024.65863668
TATTAAT73700.024.3190636
TAATCAT89200.024.2482349
TTACCTC69400.023.85320715
ATCATTA92450.023.77422311
Produced by FastQC (version 0.11.5)