FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8193739
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC47450.027.96077341
ATAGTAC48400.027.8486440
TACCTCG49250.027.14480416
TTAATCA82100.026.9402038
ATTAATC78100.026.4827677
ATTACCT67000.025.49012214
GTACCAA52450.025.42692843
TCATTAC89900.024.83635712
GTCCTAT66850.024.81652351
TATTAAT77250.024.5996116
AATAGTA60550.024.39983639
GGTCCTA57450.024.00355350
TACCAAG58100.023.918844
TTACCTC68600.023.7223915
TAATCAT93800.023.6171679
CATTACC77050.023.0737413
AAATAGT69050.022.96813838
ATCATTA99600.022.80406611
CATGCTA71650.022.76308468
GTCCCTA29900.022.7114541
Produced by FastQC (version 0.11.5)