FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10897677
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC37350.024.55037940
GTCCCTA25950.023.7441351
TAGTACC37550.023.67397941
TTAATCA71750.022.0972258
ATTAATC65950.021.3339237
TCATTAC86100.020.52814312
ATCATTA90600.020.28114911
AATAGTA46300.020.2582239
TATTAAT63650.020.238426
TAATCAT81500.019.8833169
GTCCTAT62650.019.27283151
ATTACCT57500.019.23413714
CCTATTA47500.019.1604184
CTATTAA53250.019.129285
GGTCCTA50150.018.84257750
CATGCTA67000.018.5987868
TGCTAAT64400.018.31771370
GTACCAA46750.018.19165243
ATGCTAA65700.018.16771369
AAATAGT56800.018.0550538
Produced by FastQC (version 0.11.5)